Effect of IL6 and IL23 on double negative T cells and anti ds-DNA in Systemic lupus Erythematosus Patients

Faculty of Medicine

Assiut University

Effect of IL6 and IL23 on double negative T cells and anti ds-DNA in Systemic lupus Erythematosus Patients

Research Authors
Asmaa Salah Shaltout, Douaa Sayed, Mohamed Saad Badary, Amany Mohammed Nafee,
Mona Hussein El Zohri, Rania Bakry, Shabaan Hashem Ahmed
Research Department
Department of Microbiology and Immunology
Research Journal
Human Immunology
Research Member
Mohamed Saad Badary Ali
Research Publisher
ELSEVIER
Research Rank
1
Research Vol
Vol. 77- No. 10
Research Website
https://www.ncbi.nlm.nih.gov/pubmed/27343994
Research Year
2016
Research_Pages
pp. 937-43
Research Abstract

Several evidences suggest that DN T cells, IL23 and IL6 play a role in the
pathogenesis of SLE. This study aimed to evaluate the frequency of DN T cells in
SLE patients and the relation to their activity also to assess the possible role of IL6 and IL23 on DN T cells. Thirty patients with SLE and sixteen healthy blood donorfemales were enrolled. There was a significant increase in DN T cells in patients than controls (P=0.001). These cells had a significant positive correlation with SLEDAI (r= 0.486, P= 0.006). DN T cells from SLE patient samples were expanded when stimulated in vitro with RhIL6 or RhIL23 in patients than controls. Furthermore, anti ds-DNA level was found to be increased in supernatant of PBMCs when stimulated by these cytokines in different concentrations. Our findings suggest that IL6 and IL23 may play role in SLE pathogenesis through their effect on DN T cells and anti ds-DNA.