ABSTRACT Introduction: The liver has an important role in carbohydrate metabolism since it is responsible for the balance of blood glucose levels by means of glycogenogenesis and glycogenolysis. Aim of the Study: Evaluation of the effect of the single and combined administration of insulin and N-acetyl cysteine on glucose metabolism in hepatotoxic rats. Materials and Methods: This study enrolling 5 groups of adult male albino rats. Hepatotoxicity was induced in group II, III, IV &V using carbon tetrachloride, while group I was left as control. Group II received no treatment, group III received N-acetyl cysteine, group IV received insulin while group V received insulin and N-acetyl cysteine. Samples were taken and measured liver function tests, insulin, glucose and adiponectin level as well as histopathological studies analysis of the liver specimens were measured and nonparametric multicomparison test was applied. Results: Liver function tests, group II and group IV showed higher significant statistical difference than group I and III. Also group II showed higher significant statistical difference than other groups as regarding blood glucose level and adiponectin level. The histopathological results revealed that the untreated group showed significant liver damage when compared to the control group, while treatment with NAC alone and treated with insulin and NAC showed significant reduction of all histopathological changes, whereas treatment with insulin alone significantly reduced all the histopathological changes except lobular inflammation. Conclusion: Good control of carbohydrate metabolism including insulin level, insulin resistance and adiponectin level helped in improving the prognosis of liver dysfuction diseases.