Chemical examination of the fungus Aspergillus aegyptiacus isolated from cotton textile yielded two new pyrrolidine alkaloids: aegyptolidine A (3) and aegyptolidine B (4), together with two known ophiobolin sesterterpenoids: ()-5-deoxovariecolin (1) and variculanol (2). Compound 1 is reported for the first time from a natural source. The structures of the isolated compounds were unambiguously elucidated on the basis of extensive spectroscopic data analysis (1D (1H and 13C) and 2D (1H–1H COSY, HMQC, HMBC, and NOESY)NMR, MS, IR, and UV) and comparison with literature data. Compounds 1 and 2 showed moderate cytotoxic activity against murine lymphoma cancer cell line L5178Y with ED50 7.6 and 8.1 mg/mL, respectively.