This study was carried out under laboratory conditions in Assuit insect research laboratory, plant protection research institute during the active season of 2012. Here we investigate the effect of certain different states of honey bee queens on the development of worker ovaries and hypo-pharyngeal glands (HG). These states of queens were: mated queens of 1- year-old, mated supersedure queens, virgin queens of 3- day old, queen cells, queenless and queenless/ without bee bread. Both mated queens of 1- year-old and mated supersedure queens don’t appeared any egg- laying workers (fully developed ovaries). The maximum percentage of egg- laying workers 76.75% was recorded in the workers without queens. While, the minimum percentage 3.25% was recorded in case of the workers headed with queenless/ without bee bread. All queen states under experiment occurred highly significant activation in the HG development of bee workers, in compared to queenless/ without bee bread bees. Also, the present study was showed that bee bread or protein feeding plays an important role in regulation reproductive system and food producing gland development of bee workers.