Doxorubicin is a highly effective antineoplastic agent, but it is well known for its oxidative damage to various body organs such as nephrotoxicity. The present study aimed to investigate the possible protective role of the natural antioxidant wheat germ oil on Doxorubicin -induced kidney toxicity. Studies were performed on four groups of mice. Control group, wheat germ oil group (100 mg/kg b.w.), Doxorubicin group (2.5 mg/kg/day) for seven days, and doxorubicin plus wheat germ oil group. Histopathological examination of kidney sections revealed that doxorubicin caused glomerular congestion with wide Bowman’s space and widened tubular lumen. Immunohistochemical localization of Caspase-3 for apoptosis was performed. Doxorubicin treated animals showed positive reaction to Caspase 3 in glomerulii and renal tubules as compared with controls. Administration of wheat germ oil reversed kidney damage with a marked reduction in tubular damage and apoptosis induced by doxorubicin.These results have suggested that wheat germ oil ameliorated doxorubicin -induced nephrotoxicity in male mice.