This investigation was carried out under laboratory conditions, in Assiut Insect Research laboratory, Plant Protection Research Institute, during the active season of 2012. The role of the queen in relation to comb construction in honeybees, was analyzed with respect to certain queen statuses [one-year old mated and egg-laying queens (M), three-days old virgin queens (V), supersedure queens (S), queen cells at the beginning of sealed (Q), without queens (W) and without queens / without bee bread (W/W)]. The comb construction, consisting of the numbers and including the measurements of both worker-sized and drone-sized cells, were recorded. The means of total wax cells that were constructed are, 167.00 (92.78%), 126.50 (70.28%), 138.75 (77.11%), 123.25 (68.50%), 107.25 (59.61%) and 82.75 (45.97%), for the workers under (M), (V), (S), (Q), (W) and (W/W) statuses, respectively. The constructed supernumerary of the drone-sized wax cells are 2.25 (1.78%) and 1.5 (1.22%) for the workers under (V), and (Q) statuses, respectively. In conclusion, the results showed that the comb construction (number of cells) significantly increased in queen right statuses (M); (V); (S) and (Q), compared to queenless statuses (W) and (W/W). No royal cells construction was observed in our experimental set-up with all queen statuses; so one could conclude that, the different queen statuses affecting comb construction in Apis mellifera L. workers.