Ninety isolates of Trichodema (Telomrph: Hypocrea) species and one isolate of Gliocladum viride (Tel. Hypocrea lutea) were isolated from soil samples collected from different locations in Taif city. Two soil samples cultivated with Purica granatum showed high incidences of isolates (13 and 2 isolates, respectivily). Regions f nuclear DNA, containing 18S ribosomal RNA gene (partial sequence); internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2 (complete sequence); and 28S ribosomal RNA gene (partial sequence) were amplified to identify the collected isolates. The sequencing results indicate that 78 isolates of the population were identified as Trichodema harzinum (Tel. Hypocrea lixii. Also, two isolates were identified as T. longibrachiatum (Tel. H. orientals) and one isolate Gliocladium viride (Tel. H. lutea). The remaining 10
isolates were postulated as three new species according to their phylogenic tree. T. harzianum isolates collected during this work showed high degrees of variability which supported that it is "pecies complex"