The present study aimed to evaluate in vitro anti-cancer activity of Ochradenus baccatus leaves extract
on various cell lines. The second aim determination of the total phenolic, flavonoid contents, and
antioxidant potential from the methanol extract of O. baccatus leaves collected from El-Shaikh Fadl -
Ras Gharib Road in the Eastern Desert of Egypt. The anti-proliferation activity of O. baccatus extract
was tested by SRB assay on five different cell lines: HEPG2 (liver hepatocellular carcinoma cell line),
HCT (Colon carcinoma cell line), MCF7 (breast carcinoma cell line), HEP2 (Larynx carcinoma cell line),
and H1299 (Lung carcinoma cell line). Total phenolics, antioxidants, and flavenoid contents were
determined in the extract of the xerophytic O. baccatus plant and the fluctuation of these contents due to
environmental variations. Indicated that incubation of HEP2 in the extract of leaves attained the highest
values of IC50 (47.8 μg/ml.), while the lowest (215 μg/ml.) was attained furthermore, the highest and the
lowest growth inhibition percentages (GIP) (82.81 and 54 %) were observed with HEP2 and MCF7,
respectively. Also, results showed the highest total flavenoid contents in O. baccatus leaves was during
the winter season. Summer conditions exhibited the highest contents of total phenolic compounds
accompanied by significant increases in Ca+2, Mg+2, and Na+ contents in leaves. We concluded that O.
baccatus is a rich source of polyphenolic and flavonoid compounds with good antioxidant properties that
help in the prevention and therapy of various oxidative stress-related diseases such as cancer and
hepatic diseases.