The current study was conducted to find out the influence of feeding broilers on low protein diets supplemented with prebiotic. Growth performance, carcass traits, muscle cholesterol, triglycerides, chemical composition, blood parameters and immune response of broilers were evaluated. A total number of 68 birds’ one day old broiler chicks (Ross 308) randomly distributed into 4 equal groups each of 17 chicks. The first group (control) was fed the basal (100%NRC CP) diet free from prebiotic. The other three groups (T2, T3 and T4) were fed on low protein diets (95, 90 & 85% of NRC requirements, respectively) and supplemented with prebiotic at level of 0.1%. The results showed that, birds in the second group had significantly higher live body weight, feed conversion, and dressing percentages. Supplementation of prebiotic had no significant effects on relative weight of liver, gizzard and immune organs. Reduction in the relative weight of abdominal fat pad in all prebiotic treated groups. There were significant reduction (P<0.05) in the meat cholesterol, triglycerides and fat mass of broiler breast and thigh, while protein content was significantly (P<0.05) increased in all prebiotic treated groups. A numerical increased in antibody titre in birds in the second and third groups. Birds in the second group had the best economic feed efficiency. It could be concluded that adding prebiotic to broiler diets low in protein (95, 90 and 85% of NRC) has a beneficial effect on growth parameters, carcass traits, economic value in addition to healthy and nutritious poultry products for consumers.