ABSTRACT

Objective: This study aims to evaluate the antibacterial effect and flow of a bioceramic sealer modified by different concentrations of nano magnesium hydroxide (NMH). 

Methods: NMH were characterized using transmission electron microscope and X-ray diffraction analysis. Then, added to Ceramoseal HBC by 0.025%, 0.05% and 0.1% in weight. for direct contact antibacterial test (DCT), the tested materials were added to wells of a 96-well plate. Bacterial suspension was added to each well, incubated for 1hour and 24 hours and transferred to agar plates for colony forming unit to be counted. Flow test was applied according to ISO standards and the diameters of compressed discs of tested materials were measured. The data were statistically analyzed using One way ANOVA test and Student t-test.

Results: For DCT test, all tested groups showed antibacterial effect compared to the positive control. At 1 hour, group Ⅰ showed the highest antibacterial effect. At 24 hours, group Ⅰ and Ⅳ were the same antibacterial effect that is higher than group Ⅱ and Ⅲ. Regarding flow, group Ⅰ was highest flow and group Ⅳ was the lowest but all results were more than 17 mm.

Conclusion: NMH modified sealers showed antibacterial efficacy and their flow still according to ISO requirements