This study evaluated the biocompatibility of three different root canal sealers (CeraSeal, NeoSEALER Flo, and AH-Plus) by implanting them into the subcutaneous tissues of rats and analyzing the tissue response through histopathological and immunohistochemical methods.

Materials and Methods

Sixty male Calomys callosus (Rodentia, Cricetidae) rats were used. The sealers were placed in polyethylene tubes that were inserted into certain dorsal subdermal locations. After 3, 7, and 30 days, the implants were removed, fixed, and processed for microscopic inspection using a glycol methacrylate-embedding procedure.The descriptive analysis focused on fibrous capsule thickness, severity of the inflammatory response, presence of giant cells, and evidence of biomineralization.

Results

CeraSeal and the control group exhibited a milder inflammatory response compared to NeoSEALER Flo and AH-Plus. Additionally, the control group showed the thinnest fibrous capsule, followed by CeraSeal, while AH-Plus demonstrated the thickest capsule.

Conclusions

Within the limitations of this study, CeraSeal demonstrated superior biocompatibility compared to NeoSEALER Flo and AH-Plus sealers.