This research article investigated the effect of stacking sequence of glass fibers on the notched and unnotched tensile strength of glass fibers plies reinforced epoxy matrix composites fabricated by the hand lay-up technique. The orientation of glass fabrics was kept at [(0/90)]5, [(45/-45)]5 and [(0/90), (45/-45), ]s and all the laminates were prepared using five plies for different stacking sequences with fiber volume content of 38.6 vol.% with different ratios of the specimen hole diameter to the specimen width with three different values (0.1, 0.2, 0.5). The notch sensitivity of these composites was evaluated applying Whitney-Nuismer mathematical model. The results indicated that the composites with [(0/90)]5 stacking sequence displays the highest tensile unnotched strength, whereas the composites with [±45°]5 stacking sequence displays the least strength. Moreover, the notch sensitivity of [±45°]5 composites is almost higher than those of other stacking sequences with different D/W ratios. On the other hand, the notch sensitivity of [(0/90), (45/-45), ]s composites is slightly lower than those of [(0/90)]5 composite structures for different D/W ratios. Moreover, SEM micrographs indicates the most common failure modes for [(0/90), (45/-45), ]s and [(45/-45)]5 are more significant delamination and matrix cracking than that of [(0, 90)]5.