The New Assiut Barrage and its Hydropower Plant in Egypt was initiated at May, 2012 and it is suggested to end in December, 2017.The project is considered one of the most important industrial projects that established to enhance economy and development. The project energy of about 32 MW may contribute in the main electric grid for population and developmental approaches. Surface water samples from the River Nile, around the proposed project were collected and analyzed according to EPA Method- 1669. The measured values of pH, TDS, EC, DO, COD, Total hardness, and Temperature were recorded and reached 8.3, 183.5, 317.3, 7.7, 19.5, 135, and 25.7 OC respectively and were compared with guidelines stated by the Egyptian law 48/ 1982 concerned with protection of River Nile from pollution . Biological properties were obtained and statistically analyzed and their sample locations were determined by Global Positioning System (GPS). The study helps the Environmental Impact Assessment in the stage of the operation of the hydroelectric power station.