The Aqueous extracts of red beet, China rose, henna and acid carmine stains were used to stain
the adult worms of F. gigantca. The stained flukes showed varying degrees of pigmentation with
well-defined external and internal structure. Oral and ventral sucker, muscular pharynx, oesophagus, intestinal ceca and cirrus sac took rose to light cherry color in aqueous extract of china
rose, orange to brown color in aqueous extract of sugar beet, beige to pale brown color in aqu eous extract of henna while they took deep red color in carmine stain with best quality achieved
by china rose aqueous extract. The ovary, testes and longitudinal excretory canal/pore took rose
to cherry color in aqueous extract of China rose, pale brown color in aqueous extracts of both
sugar beet and henna while they acquired red to pink color in acid carmine stain. Aqueous extracts of sugar beet exhibited little better quality in revealing characteristics of vitelline ducts and
vitelline glands compared to the aqueous extract of China rose, henna and carmine stain. Uterus
stained brown in both sugar beet aqueous extracts and carmine stain while it took yellow color
when stained with China rose and henna. Eggs inside uterus took light yellow color in all tested
aqueous extracts. Ootype/mehli’s gland and yolk reservoir of F. gigantica took brown color in
aqueous extracts of red beet, China rose and henna, red to pink color in carmine stain. Spines
covering the cuticle appeared rose color in aqueous extract of china rose, pale brown color in
aqueous extract of sugar beet extract and henna, and pale red color in carmine stain.
Key words: Fasciola gigantica, Natural herbal dye