Background Osteoporosis affects women severely, especially in older ages after menopause. It may lead to bad drawbacks that may be prone to considerable pathological conditions, for example, fracture and sever pain.
Objective This study aims to identify the effect of antioxidant and iron chelator on metabolic bone turnover in ovariectomized rats.
Materials and methods The rats were divided into five groups: negative control, positive control, desferal-treated, vitamin E-treated, and desferal+vitamin E-treated groups. Determination of serum estrogen, ferritin, alkaline phosphatase (ALP), tumor necrosis factor-α (TNF-α), and osteocalcin was done at the end of experiment. Moreover, morphology of bone in female rats was evaluated at the end of experiment.
Results Ovariectomy led to decrease in serum estrogen level and increase in serum levels of ferritin, ALP, TNF-α, and osteocalcin. On the contrary, usage of desferal and vitamin E led to decrease in serum levels of ferritin, ALP, TNF-α, and osteocalcin. Regarding histological picture of bone, our results showed abnormal structure of the bone of the untreated group vs improved histological picture of the treated groups.
Conclusion The female rats that were subjected to ovariectomy and experienced osteoporosis showed improvement in their bone by using desferal and vitamin E alone or in combination.

Keywords: bone remodeling, desferal, ovariectomy, vitamin E