ABSTRACT. Trichinellosis  is  a  serious  worldwide  parasitic  zoonosis. The  available  therapy  for  the  treatment  of
Trichinella spiralisis not satisfactory. Therefore, the recovery of effective treatment is required. This work aimed at
evaluating of the in vitro effect of silver nanoparticles (AgNPs) on muscle larvae of Trichinella. The present study
investigated the larvicidal properties of chemical and myrrh AgNPs on muscle larvae (ML) of T. spiralis. The used
AgNPs were chemically prepared using NaBH4
as reducing agentand biosynthesized using methanolic myrrh extract.
Characterization  of  synthesized  AgNPs  was  monitored  via UV-vis  spectrophotometry,  Fourier  transform  infrared
spectroscopy and transmission electron microscopy (TEM) studies. The ML incubated with AgNPs at concentrations
ranged from 1 μg/ml to 20 μg/ml. Chemical and biosynthesized AgNPs revealed marked larvicidal effect against ML of
Trichinella. Additionally, this in vitro study showed degenerative changes affecting the cuticle of AgNPs treated ML.
The  effectiveness  of AgNPs  on  the  infectivity  of TrichinellaML  was  also  assessed.  The  results  showed  complete
inhibition of the infectivity of ML exposed to sublethal doses of chemical and myrrh prepared AgNPs when used to
infect animal models. This is the first report where myrrh synthesized AgNPs have been tested for their anthelminthic
activity against Trichinellain an in vitromodel.
Keywords: Trichinella spiralis, muscular larvae, silver nanoparticles, infectivity, viability