Objectives

Detailed characterisation of CD45RA+ and CD45RO+ T cell subsets in both CD4+ and CD8+ cells in blood and tissue of patients with chronic rhinosinusitis with nasal polyps (CRSwNP) is lacking. We aimed to investigate T cell differentiation levels in blood and tissue and correlate this with CT score and white blood cell count.

Methods

This case-control study was conducted on 21 patients with CRSwNP and 20 healthy controls. Lund-Mackay score was used for radiologic staging of chronic rhinosinusitis. T cell subsets were characterised in blood and nasal polyp tissue using CD4, CD8, CCR7, CD45RA, CD45RO, CD27, and CD95 monoclonal antibodies.

Results

Most variations in T cell subsets were detected in tissue rather than blood. A higher level of CD8+ T CM, CD4+ T SCMs and lower level of CD8+ T EM were detected in the blood of CRSwNP patients vs controls. In CRSwNP patients, substantial …