Hypoxia and oxidative stress markers in pediatric patients undergoing hemodialysis: cross section study

Faculty of Medicine

Assiut University

Hypoxia and oxidative stress markers in pediatric patients undergoing
hemodialysis: cross section study

Research Authors
Enas A Hamed , Taghrid B El-Abaseri , Amany O Mohamed , Ahmed R Ahmed and Tarek H El-Metwally
Research Department
Department of Medical Physiology
Research Journal
BMC Nephrology
Research Member
Enas Ahmed Hamed Omran
Research Rank
1
Research Year
2012
Research Abstract

Background
Tissue injury due to hypoxia and/or free radicals is common in a variety of disease processes.
This cross-sectional study aimed to investigate effect of chronic kidney diseases (CKD) and
hemodialysis (HD) on hypoxia and oxidative stress biomarkers.
Methods
Forty pediatric patients with CKD on HD and 20 healthy children were recruited. Plasma
hypoxia induced factor-1α (HIF-1α), vascular endothelial growth factor (VEGF) were
measured by specific ELISA kits while, total antioxidant capacity (TAC), total peroxide
(TPX), pyruvate and lactate by enzymatic/chemical colorimetric methods. Oxidative stress
index (OSI) and lactate/pyruvate (L/P) ratio were calculated.