Summary

A method is described for measuring lipid peroxides
by means of the color reagent of a commercially available test kit
for cholesterol estimation. In principle, this assay makes use of
the oxidative capacity of lipid peroxides to convert iodide to
iodine, whic, can be measured photometrically at 365 nm. cdi-
bration curves were obtained using peroxides such as H202,t-butyl
hydroperoxide, and cumene hydroperoxide. A stoichiometric
relationship W ~ Sobserved between the amount of organic perox-
ides assayed and the concentration of iodine produced. Concen-
trations of lipid peroxides as small as 1 nmol/ml could be mea-
sured. The ability to estimate lipid pemxides of isolated low density
lipoprotein was demonstrated.