Objectives: ‎
The aim of this study is to evaluate the possible effects of selective cyclooxygenase 2 ‎inhibitor drug (celecoxib) on renal glutathione levels and interleukin 6 (IL6) ‎expressions in a gentamicin-induced nephrotoxicity model in mice. ‎
Methods:‎
‎4 groups of adult male mice (5 animals each) received saline, gentamicin (100 mg/kg/ day, i.p), celecoxib ‎‎(‎30 mg/kg/day, orally‎) and gentamycin plus celecoxib for 8 days.‎
Results:‎
It was noticed that gentamicin administration to mice led to ‎significant depletion of ‎renal glutathione, this indicates the ‎deficiency of renal antioxidant capacity by ‎gentamicin and widespread tubular damage (loss of microvilli, tubular dilatation and ‎vacuolation) in histopathological examination indicating nephrotoxicity. An ‎overexpression of IL6 was noted in renal tubular epithelium by ‎immunohistochemistry.‎
In animals received celecoxib daily, there were ‎no changes in renal glutathione levels ‎compared to control animals, no histopathological changes in renal tissues and reduced ‎expression of IL6.‎
‎In animals received celecoxib ‎plus gentamicin there is significantly reversed ‎gentamicin induced nephrotoxicity evidenced by increasing levels of glutathione, ‎reduced IL 6 expression and reduction of the degree of tubular injury by ‎histopathological examination.
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Conclusion: administration of celecoxib can protect the kidneys from gentamicin ‎related toxicity.