Moxifloxacin HCl (moxi.HCl) is a fourth generation of fluoro-quinolone which has a broad spectrum and improved anti-bacterial activity over other similar quinolones. Topical gel formulations of moxi.HCl were prepared by using gel forming agents like Carbopol 934, methyl cellulose (MC), hydroxylpropylmethylcellulose (HPMC), sodium carboxymethylcellulose (Na CMC) and sodium alginate. Compatibility studies of the drug with these polymers were performed using DSC and FT-IR techniques. Physical characterizations of moxi.HCl gels including drug content, pH measurement and rheological parameter like viscosity were studied. In vitro drug release from the prepared gel and kinetics of release were evaluated. Microbiological studies of moxi.HCl gels were carried out by using agar plate method against the tested micro-organisms. Wound healing study was performed on wound of mice infected with S.aeurus and P.aeurginosa and treated with the prepared gels. Results revealed that all the used polymers in gel preparations are compatible with moxi.HCl. All the prepared gels followed non-Newtonian (shearing thinning) pseudo-plastic flow. Higher percent cumulative drug release (87.68±2.32%) was obtained from formula (F3) containing 0.1%w/w moxi.HCl and using 4% w/v HPMC as a gel base after 8 hrs. While, formula (F5) containing 0.1 %w/w moxi.HCl and using 6%w/v of sodium alginate as a gel base showed the lowest percent cumulative drug release (50.26±1.98%) after the same time. A slight decrease in the release rate of moxi.HCl was observed by increasing the concentration of the drug to 0.5%w/w in the prepared gels. The tested formulae (F1-F5) showed a higher antibacterial activity against S.aeurus and P. aeurginosa. Formula (F3) showed a higher % of wound healing reached to 100% reduction in wound area after 6 days of topical treatment to mice with S.aeurus infected wound. Hence from the overall study, it was concluded that moxi.HCl gel would be promising in the treatment of wounds.