The current study aimed to test the neuroprotective action of topiramate in mouse peripheral diabetic neuropathy (DN) and explored some mechanisms underlying this action. Mice were assigned as vehicle group, DN group, DN + topiramate 10-mg/kg and DN + topiramate 30-mg/kg. Mice were tested for allodynia and hyperalgesia and then spinal cord and sciatic nerves specimens were examined microscopically and neurofilament heavy chain (NEFH) immunostaining was performed. Results indicated that DN mice had lower the hotplate latency time (0.46-fold of latency to licking) and lower von-Frey test pain threshold (0.6-fold of filament size) while treatment with topiramate increased these values significantly. Sciatic nerves from DN control mice showed axonal degeneration while spinal cords showed elevated GFAP (5.6-fold) and inflammatory cytokines (∼3- to 4-fold) but lower plasticity as indicated
Research Authors
Mohammed A Attia, Nema Soliman, Mohamed Ahmed Eladl, Shymaa E Bilasy, Taghrid B El-Abaseri, Howaida S Ali, Faten Abbas, Dalia Ibrahim, Noura MS Osman, Abdullah A Hashish, Asma Alshahrani, Abir S Mohamed, Sawsan A Zaitone
Research Date
Research Department
Research Journal
Toxicology Mechanisms and Methods
Research Member
Research Publisher
Taylor & Francis
Research Year
2023
Research_Pages
1-15
Research Abstract