Loop-mediated isothermal amplification assay versus polymerase chain reaction for detection of blaNDM-1 and blaKPC genes among Gram negative isolates

Faculty of Medicine

Assiut University

Loop-mediated isothermal amplification assay versus polymerase chain reaction for detection of blaNDM-1 and blaKPC genes among Gram negative isolates

Research Authors
Ayat H Mohammed, Ehsan A Hassan, Mona A Hassan, Ahmed Reda, Shrouk M Elghazaly, Shereen M Mohammed
Research Department
Department of Microbiology and Immunology
Research Journal
Microbes and Infectious Diseases
Research Member
Ayat Hussein Mohammed Mohammed
Research Year
2024
Research Abstract

Background

 New Delhi Metallo-b-lactamase (NDM- 1) and Klebsiella pneumoniae carbapenemase (KPC) are enzymes associated with resistance to many β- lactam agents. Their early detection is very important for controlling the spread of drug- resistant bacteria. This study aimed to evaluate the use of LOOP-mediated isothermal amplification technique (LAMP) assay for rapid and cost-effective detection of blaNDM-1 and blaKPC genes among Gram-negative bacteria in comparison with conventional PCR.

Methods

A total of 156 gram-negative bacterial isolates [Escherichia coli (43), Klebsiella spp. (66), Pseudomonas spp. (47)] were screened for the presence of carbapenemases (blaNDM-1 and blaKPC) using molecular methods such as conventional PCR and LAMP assay.

Results

  blaNDM1 was positive in 94/156 (60.2%) isolates and blaKPC was positive in 37/156 (23.7%) isolates by conventional PCR and LAMP.

Conclusion

The LAMP technique is an excellent option for the rapid detection of blaNDM-1 and blaKPC genes. The amplification is faster and cheaper than other molecular techniques. It is easy to implement. The thermocycler is not necessary.