Nursing care standards of colostomy are significant action to ensure quality of care. The examination of nurses’ knowledge and practices regarding the nursing care standards of colostomy has not been conducted before at Assiut University Hospital. This descriptive study aimed at establishing the level of nurses’ knowledge and practice and to examine the relationship between nurses’ knowledge and practice for developing the nursing care standards of colostomy. The subjects were nurses working in the surgical wards of hospital. The Nurses’ Knowledge Questionnaire and Practice checklist were administered. Data were analyzed by descriptive statistics and Pearson product-moment correlation. The findings revealed that all of nurses (100%) had knowledge about the standards regarding colostomy at an unsatisfactory level (M ± SD = 29.8654 ± 8.34085) with the maximum mean scores (M = 0.8269)and minimum mean scores (M =2.2308). All of nurses (100%) scored the actual nurses' practice of the standards at an unsatisfactory level (M ± SD = 111.51 ± 25.706) with the maximum mean scores (M = 19.42) and minimum mean scores (M = 2.4615). It was found that there was a positive weak relationship between knowledge and practice regarding nursing care standards of colostomy(r = 0.15, p = .3).It was concluded that nurses' knowledge and practice in the nursing care standard of colostomy were unsatisfactory. It was needed to be improved through implementation of proposed nursing care standards of colostomy.