Glaucoma is a leading cause of blindness worldwide. The study aimed to: assess employees' knowledge regarding glaucoma. Methods: Descriptive research design carried out in Assiut University. It included 1000 employees aged forty year and more. Tools: Data collected by using three tools; tool (1): included demographic characteristics, second tool included assessment of ophthalmic, medical and family history of the employees, while the third tool included assessment of knowledge regarding the glaucoma. The main results of present study were: it was found that more than 21.1% of employees were suffering from different types of eye diseases. (94.5%) of the employees had unsatisfactory knowledge regarding to glaucoma, there was a statistical significant difference between employees' level of knowledge and their education. It concluded that: The employees had unsatisfactory knowledge about glaucoma. It was recommended that: health education programs should be implemented for employees with chronic disease every year