Hepatitis C virus (HCV) is an emerging global epidemic disease. Egypt reported the highest incidence in the world. Aim of the study: To assess knowledge, attitudes and practices of barbers and their customers regarding HCV and HBV. Setting: The study carried out in Assiut District and City. Design: Descriptive research design. Sample: Included 350 participants (175 barbers and 175 customers). Tools of the study: Data collected by three tools; Tool (1): interview form included three parts; first part included demographic data; second part included assessment of medical and surgical history of barbers and customers and third part included assessment of knowledge regarding the HCV&HBV. Tool (2): Included Likert attitude scale. Tool (3): Observational checklist to assess practices of barbers and customers. Results: Majority of barbers and their customers (88.6% and 80.6% respectively) had poor knowledge regarding HCV and HBV. Conclusion: There was a statistical significant difference among customers' knowledge, their level of education and their residence. Moreover there was a significant difference between customers' attitude and their residence. Recommendations: Need for ongoing training and retraining programs for barbers regarding methods of prevention of parentally transmitted hepatitis. Increase public awareness about risk factors and prevention methods of HCV and HBV infection.