The individual with End-stage renal disease on hemodialysis is facing multiple and potentially overwhelming stressors which impact the level of social, psychological, and physical functioning. The study aimed to assess psychological stress related to hemodialysis patients. Subjects and methods: The studied sample included 250 patients; this study was conducted in El-Minia University hospital at Dialysis Unit. They were assessed through Psychological stress scale taken from Depression, Anxiety, and Stress scale. Conclusion of this study revealed that, males represent 55.2% of the studied sample, and more than half of the studied sample resided in rural area. Concerning level of stress among studied sample, 36% of patients had moderate level of stress while 14% of them suffered from severe level of stress.