Background: Persons with disabilities represent a significant portion of the world’s population and they are part of every community. They find that information on reproductive health (RH) is often inaccessible to them. Aim: evaluate the impact of an educational program about RH for blind and deaf adolescent girls. Methods: it was Quasi-experimental research design which conducted in Al Noor and Al Amal Schools in Assiut Governorate. The total sample was 80 girls. Tools: it included three tools: - a structured interview sheet, to assess disabled girls’ knowledge and attitudes towards RH and an observational checklist (using doll) to assess practice related perineal care. Results: All the studied sample (100%) has poor knowledge regarding to RH in pre test while their knowledge improved in post test to 58.8%. Regarding to perineal care, all of the studied sample (100%) carried out all steps of perineal care except three steps which include the correct direction for cleaning the perineum, while their practice improved in post and follow up test. Conclusion: All the studied girls have poor knowledge in pre test while after implementation the education programe their knowledge were improved (P= ≤ 0.001). Recommendation: Continues health education programs should be implemented for increasing awareness of them about reproductive health.