glaucoma is aleading cause of blindness worldwide. the study aimed to assess participants knowldge regarding glaucoma.methods: Quasi experimental research design carried out in Assiut University. it included 1000 participants aged forty and more. Tools: Data collected by using two tool: 1 interview form consisted of three parts: first part included demographic characteristics.the second part assessment of ophthalmic , medical and family history. third part assessment of knowledge regarding glaucoma.tool2:eye examination.done by using tonopen device, perimetry and ophthalmoscopy. the main result of present study were: 94.5% of the employees had unsatisfactory knowledge regarding glaucoma.there is was statistical significant difference between employees level of knowledge and their education. it concluded that : the employees had poor knowledge about glaucoma in pre test while after implementation the educational program their knowledge was improved. it was recommended that : periodic health education programs should be implemented for employees with chronic disease every year.