The present study aimed to; determine the quality of life of hemodialysis patients and to compare the quality of life of hemodialysis patients at Assiut University hospital and at Umulj hospital, Tabuk. Data were collected from the hemodialysis center at Assiut University hospital and at Umulj hospital, Tabuk. The study was conducted on 80 adult patients, having the following criteria; age between 18 – 65 years, both male and female patients with end stage renal disease on hemodialysis, 40 patients from each dialysis center. Data were collected through the following tools; Tool (I): Sociodemographic data sheet; including age, gender, marital status, educational level, occupation, residence, family number, duration of hemodialysis….etc. and Tool (II): World Health Organization Quality Of Life (WHOQOL); including 5 domains which are physical, social, psychological, environment, and perceived QOL. Results of this study; near half of the studied sample their age has ranged from 50 - 65 years in both groups, and more than half of the studied samples in both hospitals were having a moderate score in their quality of life. Conclusion; there was no statistically significant difference between both studied samples regarding their quality of life level, also a statistically significant relation was found between the studied groups regarding their quality of life and both patient's gender and occupation. The study recommended that; providing counseling for patient with end stage renal disease on hemodialysis will play an important role in improving the QOL of those patients.