Nursing is a stressful profession; stress is a prevalent and costly problem in today's workplace. Aim: The study aimed to assess occupational stressors and coping strategies of nurses at Assiut University Hospital. Design: A descriptive- cross sectional design was utilized in this study. Subjects and methods: The subjects included in this study 150 female nurses. The study was conducted at Assiut University Hospital at different hospital departments (general intensive care unit, medical emergency unit, burn unit, operation theatres of general and special surgery, Neurology & Psychiatry department, internal medicine department, general and special surgery departments and trumatology unit),during 6 months period from 1st January to 30th Juan 2012. Nurses were assessed through; the sociodemographic data questionnaire, in addition to socioeconomic status scale, Social readjustment rating scale, Assessment level of stress scale, Coping strategies rating scale, and Symptoms Check List (SCL-90-Revised). Results: More than half of nurses (54.7%) were from urban areas, about fifty percent 50.7% of them had more than 10 years of experience, and sixty four percent 64% of nurses had moderate level of stress. Both methods of coping strategies (emotional focused strategies and problem focused strategies) had high mean scores and very highly significant (0.006*** & 0.000***), Recommendation: A training programme is essential for nurses to provide them with a multitude of stress management techniques and coping strategies which nurses can be used to deal with their stressors and avoid the negative effect of stress on nurse' health.