Abstract:Medications use during pregnancy has always created a challenge in antenatal care due to the potential fetal risk associated with the use. The study aimed to estimate prevalence and assess attitude regarding non-prescribed medications among pregnant women attending Maternal and Child Health Care centers in Assiut City. Subjects and Methods: Design:Descriptive cross-sectional design. Setting:the current study conducted in two Maternal and Child Health Care centers.Sample: 368 pregnant women. Tool of the study: structured interview questionnaire which contained two parts: part (I): Socioeconomic scale, current gynecological and obstetric history and questions regarding using of non-prescribed medications during pregnancy (previous and current); part (II): Medications use attitude scale.Results:60.9% of pregnant women were aged from 5 to 40 years, 33.4% had secondary level of education and 79.1% of them were housewife.Also the findings observed that there was a statistically significant difference between taken non-prescribed medicationsduring the previous and current pregnancy (p=0.002). Conclusion:More than half of the pregnant women used non-prescribed medications during previous pregnancy while less than one fifth of them used it during the current pregnancyand the majority of them had positive attitude regarding medications use.Recommended: Health education campaigns through mass media should be addressing pregnant women to educate them about unsafe use of pharmaceutical products during pregnancy.