Hematuria is a life threating problem which lead to anemia and hypovolemic shock due to bleeding which affect patient life; so nurses must do great efforts to prevent complications by using urine color scale successfully to contribute a successful patients' outcomes. Aim: Evaluate the effect of teaching program about hematuria on nurses' performance at urology intensive care unit. Research design: This study was a quasi experimental design, conducted in urological intensive care unit and urological emergency unit at Assuit university hospital. Subject and method: This study was composed of 50 nurses caring patients with hematuria. Twotools were used for data collection, tool I is (knowledge assessment tool), tool II is (observational checklist). Results: The study revealed that more than half of nurses were in the age group from 20 to 25 years and held nursing technician institute, 92.0% of nurses had unsatisfactory knowledge about hematuria , but this knowledge improved after the implementation of the program. Conclusion and Recommendations: Nurses' had unsatisfactory knowledge about hematuria in pretest. The educational program was successful in achieving its goal of upgrading or improving nurse`s knowledge. A significant difference was found between scores of knowledge before and after implementing the program. The study recommends focused on establishing plans for periodical and scheduled training courses and educational programs for nurses to help in improving their knowledge.