ims of this study were to assess patient knowledge about Magnetic resonance imaging procedure, assess level of anxiety for patients undergoing Magnetic resonance imaging procedure and evaluate the effectiveness of nursing guidelines on reducing patients anxiety level for patient undergoing Magnetic resonance imaging. Material and Methods Quasi-experimental study design was utilized in this study. Hypotheses were formulated: The knowledge score for patients whom take guidelines was higher than who didn’t take and The anxiety level for patients whom take guidelines was better than who didn’t take. The Sample was of (60) adult patients from both sex who were admitted in Magnetic Resonance Imaging unit. Two tools were used: Interview patients assessment questionnaire and anxiety scale. Results showed a significant difference was found between study and control group as regard patient’s knowledge about MRI procedure in all items. A significant difference was found between study and control group regarding level of anxiety. It was concluded that, level of knowledge for patients whom take instructions and guideline was higher than whom didn't take any instructions and guideline. Level of anxiety was lower in patients whom take instructions and guideline about MRI procedure. So nursing guidelines is very necessary, which has an effect on lowering anxiety level for patient undergoing Magnetic resonance imaging. Recommendation Pre procedure teaching and instructions should be an integral part of the nurses’ duty in all hospitals. Each patient is given pamphlet include instructions about magnetic resonance imaging …