Internship nursing students are often exposed to various infections during their clinical education. Knowledge and compliance with infection control precautions are essential to prevent hospital associated infections and protect patients as well as medical workers from exposure to infectious agents. The aim of this study was to assess internship nursing students’ knowledge and practice toward infection control precautions regarding intravenous infusion. Subject: All available internship nursing students who graduated in 2019 from Faculty of Nursing, Assiut University, during their training period within 4 months. Setting: The study was conducted in ElRajhi Liver Hospital, Neurological and Psychiatric Hospital and El- Orman Heart Hospital at Assiut University Hospitals and in South Egypt Cancer Institute. Two tools were utilized for data collection; structured questionnaire and observational checklist for internship nursing students toward infection control precautions regarding IV infusion. Results: 250 internship nursing students participated in this study, most of them (86%) attended training program about infection control. This study revealed that the internship nursing students had good knowledge and adequate practice (100% and 66.8%) respectively toward infection control precautions regarding IV infusion. Conclusions: Internship nursing students under study had good level of knowledge and adequate practice about infection control precautions regarding IV infusion. Recommendations: The periodic refreshing in-service training courses should be provided to internship nursing students in order to keep them with updated knowledge and practice about infection control precautions regarding IV infusion.