Background: Urinary tract infections are among the most typical health problems during pregnancy worldwide, particularly in developing countries. By increasing pregnant women's knowledge and participation in healthy lifestyle activities, urinary tract infections should be efficiently treated with the right therapy (self-care practices).The aim: To assess pregnant women knowledge and practices towards urinary tract infection. Samples: 320 pregnant women were used as a convenient sample. Research design: This study used a descriptive cross sectional research design. Setting: Four maternal and child health centers in El-Badary city and its surrounding villages. Tools: Data were gathered using a structured interviewing questionnaire that involved three parts personal characteristics, women’s knowledge and practices regarding UTI. Results: About 61.3% of studied women had an average ,89.1% of studied women had unsatisfactory practices; statistically significant relation between total women’s knowledge and practices score (p-value <0.01) Conclusion: The results of this study shed light on gaps in pregnant women's awareness and self-care practices about UTI. Recommendation: The study suggested holding lectures on UTI and its impact on mothers and their children to enhance the success of pregnancies. Urine culture tests should be performed as a routine maternal cares from the first trimester and continues later in pregnancy. Guidelines about preventive measures of UTI for pregnant women should be providing.