Abstract Background: Cancer Prostate has been a major public health issue worldwide. The men are often diagnosed in the late stages of the disease. Increase the level of perceptions among men helps to detect and diagnostic measures early. Aim of the study: To assess perception of men's about Cancer Prostate at Main University Hospital - Assiut Governorate. Design: Descriptive research design. A convenient sample was used, the sample were included 1500 patients up to forty years who attendance in outpatients clinics, within 6 months. The study included two tools; the first tool was interview sheet to assess (a) demographic data (b) Men's perception about cancer Prostate , and the second tool was Respondents' of prostate screening testing scale . Results: More than three-fifths of the participants had low perception of cancer Prostate. Conclusion: There was a positive correlation between perceptions, & responding screening to Cancer Prostate. Recommendations: Health education programs to increase the knowledge of people regarding cancer Prostate and the importance of periodical examinations of cancer Prostate over age forty for early detections and health promotion in all its aspect