Abstract: Nutrition during pregnancy can influence the health of the mother and her newborn. Nutritional education is essential to enable antenatal mothers to understand the knowledge of nutrition to develop effective strategies to reduce malnutrition and encouraging healthier dietary behaviors. The study aimed to evaluate the effect of an educational program regarding specific nutrition for pregnant women at outpatient clinic in women’s health hospital, Assiut University. Subjects and Methods: A Quiz-experimental pre and post-test research design was used in this study. Setting: the study was conducted at the outpatient clinic in women's health hospital, Assiut University Sample: Quota sampling used in this study; the total number of pregnant women was 108. Tools of the study: which contained three tools; tool I: Socioeconomic scale developed by Abd-Eltwab, 2012, tool II included obstetrical data and tool III nutritional sheet which contained questions to assess women's knowledge about specific nutrition during pregnancy. Results: there was a statistically significant difference between the level of pregnant women’s knowledge of pre and post-test (0.000*) with mean ± SD 13.75 ± 9.66 on pre & on post-test became 38.75 ± 7.30 respectively. Conclusion: Education program leads to significant improvement in pregnant women’s knowledge regarding specific nutrition during pregnancy. Recommendation: Continuous health education programs to improve pregnant women’s knowledge toward specific nutrition during pregnancy