Background: Premarital examination is one of the health promotion activities that provided in the maternal and child health service programs. This aimed to early detection of any health problem and treatment, Preparation for marriage; providing family life education.The community health nurses play an fundamental role in providing pre marital care services that include assess hereditary risk, provide information, and appropriate helpful counseling.The study aimed to: To assess students' knowledge and attitude toward premarital examination. Subjects and Methods: Descriptive cross sectional research design was used in this study. Multistage random sample was used in this study.The study was conducted in 4 randomly selected secondary schools .The total number of sample was 879 students. Two tools were used in this study the first tool included personal characteristics and student's knowledge , while the second tool lickert scale toward premarital examination. Results: regarding knowledge 66.0% of studied students had poor score of knowledge while 50.4% of them had negative attitude toward premarital examination. Conclusion: A significant gap in student's knowledge regarding premarital examination, while about half of them had positive attitude ,also there is positive Correlation between total score of students knowledge and attitude about premarital examination. Recommendation: Conducting counseling sessions with social workers in schools to convey information to students about premarital examination