قسم البحث
مجلة البحث
Angew. Chem. Int. Ed., DOI: 10.1002/anie.200803906
تصنيف البحث
1
عدد البحث
Vol. 47
سنة البحث
2008
المشارك في البحث
ملخص البحث
Enzyme in action: Labeling studies and the finding that carboxymethylproline synthase catalyzes production of deuterated (2S,5S)-6,6′-dimethyl-trans-carboxymethylproline (3) from dimethylmalonyl-CoA (1) and labeled l-pyrroline-5-carboxylate (2) limit possible mechanisms of C-C bond formation and thioester hydrolysis. A key feature in the catalysis is that intermediates are stabilized by hydrogen bonds in the “oxy-anion hole” of the enzyme (dark curve in scheme).