A new, simple, and stability indicating thin layer chromatographic (TLC) method has been developed for estimation of norfloxacin. Study was performed on precoated silica gel F254 TLC plate using environmentally safe mobile phase consisting of n-butanol : methanol : ammonia (33%) (8:1:3.5 v/v). A Camag TLC Scanner 3 set at 282 nm was used for direct determination of the chromatograms in the reflectance / absorbance mode. Method was validated according to ICH guidelines. Determination coefficient of the calibration curve was 0.9962 in the range 6–150 ng/band with limit of detection and limit of quantification of 1.01 ng/band and 3.06 ng/band, respectively. Statistical comparison of the results with those of the official HPLC method showed good agreement between the official and the proposed methods with respect to accuracy and precision. Norfloxacin was subjected to forced degradation at 150°C with 2M HCl or 2M NaOH, and the degradation rate in both cases was studied. The calculated first order rate constant for the degradation were determined at 0.056 and 0.023 hour1 and the t½ were 12.28 and 30.09 hr for acid and base degradation, respectively. The method has the potential to determine norfloxacin in different dosage forms and in quality control laboratories.