The Ziziphus species (Rhamnaceae family) are considered to be multipurpose plants and have been used as foods, folklore medicines, the environmental protection plants. This study was conducted to isolated and evaluate the antimicrobial activity of ethanol extract of Ziziphus jujuba seeds against sixe bacterial strains by determining minimum inhibitory concentration (MIC) and analyzed their content by using chromatographic techniques to identify the principal bioactive phytochemicals. GC/MS analysis of ethanol extract of Ziziphus jujuba seed revealed the existence of 20 component, main components were 13-Heptadecyn-1-ol (12.95%), 7-Ethyl-4-decen-6-one (9.73%), Lineoleoyl chloride (8.54%), Linoleic acid (6.37%), 2,5-Octadecadiynoic acid, methyl ester (5.57%) and Palatinol A (4.81%). The results indicated that the ethanolic extract of Ziziphus jujuba seed contains a many bioactive components that could have advantage offer a platform of using Ziziphus jujuba seed as herbal alternative for the current synthetic antimicrobial agents.