Chemical investigation of the methanolic extract of the rhizomes of
Iris germanica L. (Iridaceae) afforded two new compounds; irigenin S (7) and iriside A (12),
together with ten known compounds: stigmasterol (1), -irone (2), γ-irone (3),
3-hydroxy-5-methoxyacetophenone (4), irilone (5), irisolidone (6), irigenin (8),
stigmasterol-3-O-β-D-glucopyranoside (9), irilone 4'-O--D-glucopyranoside (10) and
iridin (11). Their structures were established by UV, IR, 1D (1H and 13C) and 2D
(1H-1H COSY, HMQC, and HMBC) NMR spectroscopy, in addition to mass spectroscopic
data and comparison with literature data. The methanolic extract was evaluated for its
antimicrobial activity. Both the methanolic extract and the isolated flavonoids were tested
for their anti-inflammatory activity.