A simple, sensitive, novel and eco-friendly electrochemical sensor was fabricated using naturally occurring dye and glassy carbon microparticles as an electro-conducting modifier. The modified electrode was characterized by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and scanning electron microscopy (SEM) techniques. This novel electrochemical sensor gave potent electrocatalytic activity and lowest detection limit toward oxidation of dacarbazine (DAC). The proposed method showed good linearity in the range of 7×10−8 to 5×10−6 M with low detection limit of 1.28×10−8 M. Furthermore, different chemical and instrumental variables were studied by factorial design approach and the optimum analytical conditions for determination of DAC were established. The proposed method was successfully applied to study photolytic degradation of DAC in 5% dextrose and 0.9% sodium chloride infusions after exposure to daylight and UV light. The presented work suggested different photo-degradation pathways of DAC indicating its importance for future development of a stable formulation for a long-term infusion therapy.