Background: Sitagliptin (SITA), is an oral anti-diabetic drug of dipeptidyl peptidase-4 (DPP-4) inhibitor class used as a monotherapy or in association with metformin to treat type II Diabetes Mellitus.
Methods: Two new, sensitive and precise spectrofluorimetric methods have been developed for the determination of SITA in tablets and spiked human urine samples. SITA has a native fluorescence that was enhanced by 2 folds after addition of β-cyclodextrin (method A). The fluoregenic product was measured at 296 nm after excitation at 267 nm. In addition, SITA was derivatized by 7-chloro-4- nitrobenzofurazon (NBD-Cl) to a highly sensitive fluoregenic product was measured at 532 nm after excitation at 466 nm (method B).
Results: The fluorescence intensities were directly proportional to the concentration over the range 0.35-10 µg mL-1 and 0.1-3 µg mL-1 for method A and B, respectively. The %RSD for inter-day and intra-day precisions was in a range of 0.160-0.915% and the accuracy results were in the range of 96.28% -100.03%.
Conclusion: Successful applications of the developed methods, for drug determination in pharmaceutical tablet and spiked human urine samples, were performed with high accuracy and precision.
قسم البحث
مجلة البحث
Current Pharmaceutical Analysis, DOI:10.2174/1573412913666170906164249
الناشر
NULL
تصنيف البحث
1
عدد البحث
Vol 14
موقع البحث
NULL
سنة البحث
2018
المشارك في البحث
ملخص البحث