The enhancement effect of reduced graphene oxide (rGO) combined with silver nanocomposite supported on poly brilliant blue (PBB) platform was investigated for ultra-trace analysis of rosuvastatin (RS). Herein, in situ electrochemical deposition of the silver nanoparticles (AgNPs) and rGO hybrid was performed on the surface of the polymerized brilliant blue (PBB) platform. The developed (AgNPs–rGO/PBB) electrode showed an enhanced catalytic activity toward the oxidation of RS. The modified electrodes AgNPs–rGO/PBB and AgNPs/PBB required an overpotential of 0.68 and 1.06 V to achieve a current density of 10 mA cm2, and their corresponding Tafel slopes were calculated to be 70 and 105 mV dec1, respectively. Further, rGO and silver nanocomposites properties were characterized by scanning electron microscopy (SEM), cyclic voltammetry (CV), square wave voltammetry (SWV), linear sweep voltammetry (LSV), and electrochemical impedance spectroscopy (EIS). Additionally, the formation of GO and AgNPs–rGO was confirmed by Raman spectroscopy and Fourier transform infrared spectroscopy (FT-IR). Under the optimized conditions, the electrochemical sensor showed a remarkable response for quantitation of RS over a wide range of concentrations 5x109 to 5x107 mol L1 (r ¼ 0.9988), with a limit of detection 2.17x109 mol L1. The electrochemical performance of the studied electrode showed high reproducibility and suitability for tablets and human plasma.