A novel selective and reliable ratiometric fluorescence probe has been successfully synthesized for precise, sensitive, and simple quantitation of methotrexate (MTX). Hydrothermal method was employed to fabricate nitrogen-doped carbon dots using Annona squamosa seeds (AS-CDs) as a starting material, and their characteristics were confirmed using transmission electron microscopy (TEM), UV-Vis spectroscopy, fluorescence spectroscopy, X-ray diffractometry (XRD), and Fourier Transform Infrared Spectroscopy (FTIR). The ratiometric fluorometric assay, which is based on measuring the ratio of emissions (F355/F430), has a wide detection range of 5–2000 ng /mL and a limit of detection (LOD, S/N = 3) of 1.5 ng /mL. The developed sensing method was successfully applied to the quantification of MTX in rabbit plasma samples and parenteral formulations, achieving satisfactory recoveries %. Magnetic molecularly imprinted solid-phase microextraction was used for selective extraction of MTX from plasma samples. The pharmacokinetic parameters were successfully determined in real rabbit plasma samples after intravenous administration of MTX. The as-designed probe does not only improve the sensitivity, but also enhances the precision and accuracy of the proposed method. Overall, this study presents a promising approach for the detection of MTX in genuine samples with acceptable degree of selectivity and sensitivity.
تاريخ البحث
قسم البحث
مجلة البحث
Journal of Pharmaceutical and Biomedical Analysis
الناشر
ElSevier
عدد البحث
238
موقع البحث
https://www.sciencedirect.com/science/article/pii/S0731708523006313
سنة البحث
2024
المشارك في البحث
صفحات البحث
115862
ملخص البحث