For the first time, we introduce a new disposable and ultrasensitive sensing electrode made up of nanostructured gold anchored porous carbon black (Au@PCB) sol–gel nanoceramic conductive film (CF) coated eco-friendly and inexpensive pencil graphite rods (PGRs) to explore their electro-catalytic sensing of dopamine (DA) in the absence and presence of uric acid (UA). X-ray diffraction (XRD) and scanning electron microscopy combined with energy-dispersive X-ray spectroscopy (SEM-EDX) were used for evaluating the Au@PCB nanocomposite. The CV analysis revealed that the disposable Au@PCB-CF/PGRE sensor exhibited outstanding redox activities in the optimal probe [Fe(CN)₆]^3−/4−. The conductive Au@PCB nanocomposite exhibits exceptional electrochemical characteristics, a larger electrochemically active surface area, and remarkable electro-catalytic activity. As a result, the oxidation current of