Monitoring the Activity of 2-Oxoglutarate Dependent Histone Demethylases by NMR Spectroscopy: Direct Observation of Formaldehyde

Faculty of Pharmacy

Assiut University

Monitoring the Activity of 2-Oxoglutarate Dependent Histone Demethylases by NMR Spectroscopy: Direct Observation of Formaldehyde

Research Authors
Richard J. Hopkinson, Refaat B. Hamed, Nathan R. Rose, Timothy D. W. Claridge, Christopher J. Schofield
Research Department
Department of Pharmacognosy
Research Journal
ChemBioChem., DOI: 10.1002/cbic.200900713
Research Rank
1
Research Vol
Vol. 11
Research Year
2010
Research Member
Refaat Hamed
Research Abstract

Formaldetected: Application of 1H- and 1D 13C HSQC NMR spectroscopy methods for monitoring the activity of a 2-oxoglutarate dependent histone Nε-methyllysine demethylase (JMJD2E) enabled the in situ quantitative analysis of the different components of the reaction, including the direct observation of hydrated formaldehyde resulting from enzyme-catalysed demethylation.