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Chromatographic Determination of Aliphatic Aldehydes in Human Serum After Pre-Column Derivatization Using 2,2'-furil, a Novel Fluorogenic Reagent

Research Authors
Marwa Fathy Bakr Ali, Naoya Kishikawa, Kaname Ohyama, Horria Abdel-Mageed Mohamed, Hanaa Mohamed Abdel-Wadood, Ashraf Mohamed Mohamed, Naotaka Kuroda
Research Journal
Journal of Chromatography A, http://dx.doi.org/10.1016/j.chroma.2013.03.033
Research Rank
1
Research Vol
vol.1300
Research Year
2013
Research Member
Research Abstract

A novel, highly sensitive and selective fluorimetric liquid chromatographic method for simultaneous determination of medium chain aliphatic aldehydes was developed. The method was based on the derivatization of aliphatic aldehydes with 1,2-di(2-furyl)-1,2-ethanedione (2,2'-furil), a novel fluorogenic reagent, to form highly fluorescent difurylimidazole derivatives. The fluorescence derivatives were separated in less than 20 min on a reversed-phase ODS column using an isocratic elution with a mixture of methanol-water (80:20, v/v%). The detection limits were from 0.19 to 0.50 nM (1-10 fmol/injection) at a signal-to-noise ratio (S/N) of 3. This method was successfully applied for monitoring of aliphatic aldehydes in healthy human sera by a simple pretreatment procedure without interferences from serum constituents.